Journal: Genes

Article Title: Matrix Metalloproteinase Genes ( MMP1, MMP10, MMP12 ) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures

doi: 10.3390/genes11070766

Figure Lengend Snippet: Minor allele frequencies (MAF) and Hardy-Weinberg equilibrium probabilities for the investigated variants (state variants) in the control and anterior cruciate ligament rupture groups investigated in the recruited Polish cohort.

Article Snippet: All samples were genotyped in duplicate, using C__34384693_10 (for the MMP1 rs1799750, -/G), C____632734_20 (for the MMP10 rs486055, C/T) and C__15880589_10 (for the MMP12 rs2276109, T/C) TaqMan ® Pre-Designed SNP Genotyping Assays (Applied Biosystems) on a StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument (Applied Biosystems, Waltham, MA, USA) following the manufacturer’s recommendations.

Techniques: Control

Journal: Genes

Article Title: Matrix Metalloproteinase Genes ( MMP1, MMP10, MMP12 ) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures

doi: 10.3390/genes11070766

Figure Lengend Snippet: Association analysis of the MMP1 rs1799750 -/G, MMP10 rs486055 C/T and MMP12 rs2276109 T/C polymorphisms with non-contact anterior cruciate ligament (ACL) rupture.

Article Snippet: All samples were genotyped in duplicate, using C__34384693_10 (for the MMP1 rs1799750, -/G), C____632734_20 (for the MMP10 rs486055, C/T) and C__15880589_10 (for the MMP12 rs2276109, T/C) TaqMan ® Pre-Designed SNP Genotyping Assays (Applied Biosystems) on a StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument (Applied Biosystems, Waltham, MA, USA) following the manufacturer’s recommendations.

Techniques:

Journal: Genes

Article Title: Matrix Metalloproteinase Genes ( MMP1, MMP10, MMP12 ) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures

doi: 10.3390/genes11070766

Figure Lengend Snippet: Association analysis of the MMP1 x MMP10 interaction with non-contact ACL rupture (codominant model).

Article Snippet: All samples were genotyped in duplicate, using C__34384693_10 (for the MMP1 rs1799750, -/G), C____632734_20 (for the MMP10 rs486055, C/T) and C__15880589_10 (for the MMP12 rs2276109, T/C) TaqMan ® Pre-Designed SNP Genotyping Assays (Applied Biosystems) on a StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument (Applied Biosystems, Waltham, MA, USA) following the manufacturer’s recommendations.

Techniques:

Journal: Genes

Article Title: Matrix Metalloproteinase Genes ( MMP1, MMP10, MMP12 ) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures

doi: 10.3390/genes11070766

Figure Lengend Snippet: Association analysis of the MMP1 x MMP12 interaction with non-contact ACL rupture (codominant model).

Article Snippet: All samples were genotyped in duplicate, using C__34384693_10 (for the MMP1 rs1799750, -/G), C____632734_20 (for the MMP10 rs486055, C/T) and C__15880589_10 (for the MMP12 rs2276109, T/C) TaqMan ® Pre-Designed SNP Genotyping Assays (Applied Biosystems) on a StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument (Applied Biosystems, Waltham, MA, USA) following the manufacturer’s recommendations.

Techniques:

Journal: Genes

Article Title: Matrix Metalloproteinase Genes ( MMP1, MMP10, MMP12 ) on Chromosome 11q22 and the Risk of Non-Contact Anterior Cruciate Ligament Ruptures

doi: 10.3390/genes11070766

Figure Lengend Snippet: Haplotype-based association of MMP1 , MMP10 and MMP12 polymorphisms (rs1799750, rs486055, rs2276109) with non-contact ACL rupture.

Article Snippet: All samples were genotyped in duplicate, using C__34384693_10 (for the MMP1 rs1799750, -/G), C____632734_20 (for the MMP10 rs486055, C/T) and C__15880589_10 (for the MMP12 rs2276109, T/C) TaqMan ® Pre-Designed SNP Genotyping Assays (Applied Biosystems) on a StepOne Real-Time Polymerase Chain Reaction (RT-PCR) instrument (Applied Biosystems, Waltham, MA, USA) following the manufacturer’s recommendations.

Techniques:

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CCN1 secretion and cleavage regulate the lung epithelial cell functions after cigarette smoke

doi: 10.1152/ajplung.00102.2014

Figure Lengend Snippet: CSE-induced CCN1 augments matrix metalloproteinase (MMP)-1 secretion. A: effects of CCN1 knockdown on MMP1. Beas2B cells were treated with control or CCN1 siRNA, followed with 10% CSE. MMP1 levels in supernatants were determined using ELISA 4 h later. B: the soluble factor and extracellular vehicles (EVs) were isolated from CSE-stimulated Beas2B cells. Beas2B cells were treated with control or CCN1 siRNA. The Beas2B cells were stimulated by the soluble factor (10 μg) and EV (10 μg). MMP1 was then determined using ELISA. C: Beas2B cells were treated with recombinant CCN1 (1 μg/ml), plasmin (0.1 μg/ml), and CCN1 + plasmin. After 8 h, MMP1 was determined using ELISA. CCN1 and plasmin were preincubated 1 h at 37°C before adding to the cells. D: Beas2B cells were pretreated for 1 h with TLCK before CSE, and MMP1 was determined using ELISA 8 h later. E: level of MMP1 after treatment of NH2-terminal or COOH-terminal CCN1 fragments (each 1 μg) in Beas2B cells. Supernatant (Sup) was taken after 24 h and measured MMP1. F: cell survival after treatment of NH2-terminal or COOH-terminal CCN1 fragments for 48 h in Beas2B cells. Cell survival was measured by CellTiter-Glo Luminescent cell viability assay. *P < 0.05 compared with control for A and B, sham for C and F, and CSE for D and E. **P < 0.05 compared with CCN1 and plasmin for C and NH2-terminal for E. Each panel represents at least 3 repeats with similar results.

Article Snippet: The human IL-8 ELISA kit was purchased from Thermo (Rockford, IL), and human VEGF and MMP-1 duoset was from R&D Systems and followed the manufacturer's instructions. .

Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Recombinant, Cell Viability Assay

Journal: International Journal of Molecular Sciences

Article Title: Proteoglycan Combined with Hyaluronic Acid and Hydrolyzed Collagen Restores the Skin Barrier in Mild Atopic Dermatitis and Dry, Eczema-Prone Skin: A Pilot Study

doi: 10.3390/ijms221910189

Figure Lengend Snippet: Variations in the expression levels of tumor necrosis factor ( TNF) –α expression between the negative control, 1 μM Dexamethasone treatment and 0.05% H.ECM TM liposome groups compared to the LPS-treated RAW 264.7 cells ( A ) Expression levels of COL1A1 and MMP1 genes. The expression levels of COL1A1 ( B ) and MMP1 ( C ), representing the expression of wrinkle-related factors, improved with 0.05% H.ECM TM liposome treatment compared to the negative control group of HDF cells ( B ). The 10 ng/mL recombinant EFG treatment was used as a positive control. * p < 0.05, ** p < 0.01, *** p < 0.005.

Article Snippet: The synthesized cDNA, Taqman Gene Expression Master Mix (Applied Biosystems), and Taqman primer of each target ( HAS3 ; Hs00193436_m1, AQP3 ; Hs00185020_m1, COL1A1 : Hs00164004_m1, MMP1 : Hs00899658_m1; Filaggrin ; Hs00856927_g1, Loricrin ; Hs01894962_s1, Applied Biosystems) were used for the qRT-PCR experiments.

Techniques: Expressing, Negative Control, Recombinant, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Proteoglycan Combined with Hyaluronic Acid and Hydrolyzed Collagen Restores the Skin Barrier in Mild Atopic Dermatitis and Dry, Eczema-Prone Skin: A Pilot Study

doi: 10.3390/ijms221910189

Figure Lengend Snippet: Expression levels of filaggrin , loricrin , HAS3, and AQP3 genes in untreated tissue specimen (negative control), and only UVB irradiated (UV), 0.1% (UV + 0.1%) and 0.2% (UV + 0.2%) H.ECM TM liposome treatment in UVB-irradiated tissue specimens. Expressions of genes encoding the factors related to the skin barrier function such as the filaggrin and loricrin were induced in the 0.1% and 0.2% H.ECM TM liposome treatment groups more than the UV group ( A ). The expression levels of genes COL1A1 and MMP1 encoding skin hydration factors were higher in the 0.1% and 0.2% H.ECM TM liposome treatments than the UV group ( B ). * p < 0.05, ** p < 0.01, *** p < 0.005.

Article Snippet: The synthesized cDNA, Taqman Gene Expression Master Mix (Applied Biosystems), and Taqman primer of each target ( HAS3 ; Hs00193436_m1, AQP3 ; Hs00185020_m1, COL1A1 : Hs00164004_m1, MMP1 : Hs00899658_m1; Filaggrin ; Hs00856927_g1, Loricrin ; Hs01894962_s1, Applied Biosystems) were used for the qRT-PCR experiments.

Techniques: Expressing, Negative Control, Irradiation